12 Cell viability is assessed by using
a) MTT
b) FCS
c) Trypan Blue
d) Tetrazolium
Category: Science homework help
Ficol-Hypaque or Lymphoprep
11 RBC and WBC can be separated based on density centrifugation by using
a) MTT
b) Ficol-Hypaque or Lymphoprep
c) Lympholysis
d) Trypan blue
Staining with Trypan Blue
10 Positive selection of T cells can be achieved by using the following antibody coated beads
a) Anti-CD3 coated beads
b) Anti-CD19 coated
c) Anti-CD14 coated beads
d) Staining with Trypan Blue
final cell concentration
9 cell counts were 1600; dilution factor was 2; final cell concentration was 3.2X10 7 /ml. how many
quadrants were used for cell counts?
a) 3
b) 4
c) 2
d) 1
main ingredients in complete RPMI
8 The following are main ingredients in complete RPMI used for cell culture
a) 10% FCS; 1% Glutamine; 1% P/S (penicillin and streptomycin)
b) 1% FCS; 10% Glutamine; 12% P/S
c) 20% FCS; 1% Glutamine; 15% P/S
d) None of the above
hemocytomer and cells were counted.
7 Peripheral blood lymphocyte cell samples were placed in hemocytomer and cells were counted.
Total Cell counts 120. Following multiplication with the dilution factor 10, cell concentration was
6X10 6 /ml. Cells were counted from how many quadrants?
a) 4
b) 3
c) 2
d) 1
incubate with MTT and culture
6 Show the correct order for MTT assay to assess cell proliferation
a) Prepare cells, incubate with MTT and culture for 3 days and read at 550-600
b) Prepare cells, incubate with detergent, culture, add MTT, read at 550-600
c) Culture cells for 72 hours, add MTT, add detergent, read at 550-600
d) Culture with MTT and detergent for 72 hours, read at 550-600
Trypan blue stains
5 Trypan blue stains viable cells
a) True b) False
cell counting using hemocytometer
4. Following cell isolation and cell counting using hemocytometer, you obtained 6X10 6 /ml ( 6 million
cells per milliliter). Total volume you have is 1 ml.
You are asked to set up cell cultures as follows: 1) control cells without any stimulant, 2) cells with
stimulant A, 3) cells with stimulant B, 4) cells with both stimulant A and B. Total four conditions.
You are asked to use 1.5X10 6 /ml per condition to set up the experiment.
Therefore, you have to dilute the cells you obtained after counting (6X10 6 /ml).
To prepare 1.5X10 6 /ml per condition, how much medium do you have to add to your 6X10
6 /ml.
EXPLAIN: SHOW THE WORK OUT DETAILS
cell concentration
3 peripheral blood lymphocyte cell samples were placed in hemocytomer and cells were counted in 3
quadrants. Total Cell counts in 3 quadrants were 80. Following final calculations cell concentration was
2.6X10 6 /ml. What is the dilution factor? Please show how you calculated?
a) Dilution factor is 5
b) Dilution factor is 2
c) Dilution factor if 10
d) Dilution factor is 20