12 Cell viability is assessed by using
a) MTT
b) FCS
c) Trypan Blue
d) Tetrazolium
12 Cell viability is assessed by using
a) MTT
b) FCS
c) Trypan Blue
d) Tetrazolium
11 RBC and WBC can be separated based on density centrifugation by using
a) MTT
b) Ficol-Hypaque or Lymphoprep
c) Lympholysis
d) Trypan blue
10 Positive selection of T cells can be achieved by using the following antibody coated beads
a) Anti-CD3 coated beads
b) Anti-CD19 coated
c) Anti-CD14 coated beads
d) Staining with Trypan Blue
9 cell counts were 1600; dilution factor was 2; final cell concentration was 3.2X10 7 /ml. how many
quadrants were used for cell counts?
a) 3
b) 4
c) 2
d) 1
8 The following are main ingredients in complete RPMI used for cell culture
a) 10% FCS; 1% Glutamine; 1% P/S (penicillin and streptomycin)
b) 1% FCS; 10% Glutamine; 12% P/S
c) 20% FCS; 1% Glutamine; 15% P/S
d) None of the above
7 Peripheral blood lymphocyte cell samples were placed in hemocytomer and cells were counted.
Total Cell counts 120. Following multiplication with the dilution factor 10, cell concentration was
6X10 6 /ml. Cells were counted from how many quadrants?
a) 4
b) 3
c) 2
d) 1
6 Show the correct order for MTT assay to assess cell proliferation
a) Prepare cells, incubate with MTT and culture for 3 days and read at 550-600
b) Prepare cells, incubate with detergent, culture, add MTT, read at 550-600
c) Culture cells for 72 hours, add MTT, add detergent, read at 550-600
d) Culture with MTT and detergent for 72 hours, read at 550-600
5 Trypan blue stains viable cells
a) True b) False
4. Following cell isolation and cell counting using hemocytometer, you obtained 6X10 6 /ml ( 6 million
cells per milliliter). Total volume you have is 1 ml.
You are asked to set up cell cultures as follows: 1) control cells without any stimulant, 2) cells with
stimulant A, 3) cells with stimulant B, 4) cells with both stimulant A and B. Total four conditions.
You are asked to use 1.5X10 6 /ml per condition to set up the experiment.
Therefore, you have to dilute the cells you obtained after counting (6X10 6 /ml).
To prepare 1.5X10 6 /ml per condition, how much medium do you have to add to your 6X10
6 /ml.
EXPLAIN: SHOW THE WORK OUT DETAILS
3 peripheral blood lymphocyte cell samples were placed in hemocytomer and cells were counted in 3
quadrants. Total Cell counts in 3 quadrants were 80. Following final calculations cell concentration was
2.6X10 6 /ml. What is the dilution factor? Please show how you calculated?
a) Dilution factor is 5
b) Dilution factor is 2
c) Dilution factor if 10
d) Dilution factor is 20